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Properties

Assay Details
Status
17 Common Properties
 Project
4DN
 Lab
Arjun Raj, UPENN
 Experiment Category
Microscopy
 Experiment Type
RNA FISH
 Organism
Mouse
 Sample Type
stem cells
 Sample Tier
Unclassified
 Sample Category
Mouse stem cell
 Sample
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
 Organ/System
No value
 Tissue Source
stem cell
 Publication
Bartman CR et al. (2018) PMID:30554946
 Modifications
No value
 Treatments
Chemical
 Center
NT - Raj
 Commendations
No value
 Warnings
Biosample Metadata Incomplete
Title
Experiment Type
Biosample
Dataset
Condition
Assay Details
Lab
RNA FISH
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
nascent transcript RNA FISH - flavopiridol treatment
prdx2, slc25a37 probes, 1000nM flavo
Target
Chromosomes, GRCm38:8:84969622-84974811 mouse region, GRCm38:14:69241851-69285103 mouse region
RNA FISH
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
nascent transcript RNA FISH - flavopiridol treatment
prdx2, slc25a37 probes, 100nM flavo
Target
Chromosomes, GRCm38:8:84969622-84974811 mouse region, GRCm38:14:69241851-69285103 mouse region
RNA FISH
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
nascent transcript RNA FISH - flavopiridol treatment
prdx2, slc25a37 probes, 10nM flavo
Target
Chromosomes, GRCm38:8:84969622-84974811 mouse region, GRCm38:14:69241851-69285103 mouse region
RNA FISH
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
nascent transcript RNA FISH - flavopiridol treatment
prdx2, slc25a37 probes, control
Target
Chromosomes, GRCm38:8:84969622-84974811 mouse region, GRCm38:14:69241851-69285103 mouse region
RNA FISH
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
nascent transcript RNA FISH - flavopiridol treatment
hbb-b1, tal1, slc4a1 probes, 1000nM flavopiridol
Target
Chromosomes, GRCm38:7:103826534-103827928 mouse region, GRCm38:4:115056426-115071755 mouse region, GRCm38:11:102348820-102365281 mouse region
RNA FISH
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
nascent transcript RNA FISH - flavopiridol treatment
hbb-b1, tal1, slc4a1 probes, 100nM flavopiridol
Target
Chromosomes, GRCm38:7:103826534-103827928 mouse region, GRCm38:4:115056426-115071755 mouse region, GRCm38:11:102348820-102365281 mouse region
RNA FISH
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
nascent transcript RNA FISH - flavopiridol treatment
hbb-b1, tal1, slc4a1 probes, 10nM flavopiridol
Target
Chromosomes, GRCm38:7:103826534-103827928 mouse region, GRCm38:4:115056426-115071755 mouse region, GRCm38:11:102348820-102365281 mouse region
RNA FISH
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion
nascent transcript RNA FISH - flavopiridol treatment
hbb-b1, tal1, slc4a1 probes, control
Target
Chromosomes, GRCm38:7:103826534-103827928 mouse region, GRCm38:4:115056426-115071755 mouse region, GRCm38:11:102348820-102365281 mouse region
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