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10 fewer sets in 4DN-only Data.
 

Properties

Sample Type
Sample Category
Sample
Organ/System
Tissue Source
Treatments
Assay Details
Warnings
11 Common Properties
 Project
External
 Lab
Alistair Boettiger, STANFORD
 Experiment Category
Microscopy
 Experiment Type
multiplexed FISH
 Organism
Human
 Sample Tier
Unclassified
 Publication
Bintu B et al. (2018) PMID:30361340
 Modifications
No value
 Center
External
 Status
Released
 Commendations
No value
Title
Experiment Type
Biosample
Dataset
Condition
Assay Details
Lab
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:34-37 Mb in HCT116 cells cohesin-depleted after cell division
Target
83 consecutive 30-kb loci on chr21, Geminin, EdU labeled Chromosomes
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:34-37 Mb in HCT116 cells cohesin-depleted
Target
83 consecutive 30-kb loci on chr21
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:34-37 Mb in HCT116 cells
Target
83 consecutive 30-kb loci on chr21
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in HCT116 cells cohesin-depleted
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in HCT116 cells
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
A549
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in A549 cells
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
K562
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in K562 cells
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
IMR-90
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in IMR90 cells across cell cycle
Target
65 consecutive 30-kb loci on chr21, Geminin, Chromosomes
multiplexed FISH
IMR-90
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in IMR90 cells
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
IMR-90
Chromatin tracing of 2-Mb on chr21
Chr21:18-20 Mb (no TADs) in IMR90 cells
Target
65 consecutive 30-kb loci in a region without TADs on chr21
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