Biosample

4DNBSGICXME4
released
View JSON

CRISPR/cas9-modified Patski cells (Del-hinge clone a) with mock treatment 1 prepared for RNA-seq; biological replicate 1

   February 6th, 2018 at 9:51pm

Overview

Stable Genomic Modifications 
Crispr deletion of 127 kb 'hinge' region near Dxz4 gene
Treatment 
None
Biosample Protocols 
Biosample preparation protocol from 2017-12-15

Biosource

Biosource 
Patski - 4DNSRJ2LKIV4
Biosource Type 
Immortalized cell line
Cell Line Name 
Patski
Individual 
4DNINWJ51C9L
Biosource Vendor 
Disteche Lab

Cell Culture

Cell Culture Information 
BiosampleCellCulture from 2018-02-06
Description 
Patski cells are cultured in DMEM (Dulbecco's Modified Eagle Medium) with 10% FBS and 1% penicillin/streptomycin. Mock treatment with 0µM 5-aza-2’-deoxycytidine (5-aza-2dC)
Synchronization Stage 
None
Morphology Image 
None
Total Days in Culture 
3
Culture Start Date 
June 26th, 2016 12:00am