Biosample

4DNBS8Q2TT5U
released

CRISPR/cas9-modified Patski cells (Del-hinge clone a) with 4uM 5-aza treatment prepared for RNA-seq; biological replicate 1

   February 6th, 2018 at 9:51pm

Overview


Biosource


Biosource Type 
Immortalized cell line
Cell Line Name 
Individual 
Biosource Vendor 

Cell Culture


Cell Culture Information 
Description 
Patski cells are cultured in DMEM (Dulbecco's Modified Eagle Medium) with 10% FBS and 1% penicillin/streptomycin. To induce DNA demethylation a 48h treatment using 4µM 5-aza-2’-deoxycytidine (5-aza-2dC) was used, followed by a 24h recovery period.
Synchronization Stage 
None
Morphology Image 
None
Total Days in Culture 
3
Culture Start Date 
June 26th, 2016 12:00am