BiosampleCellCulture from December 3rd, 2023

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Neural precursor cell differentiation of F121-6 day11 EB cells followed by single NPC cloning, expansion and storage in freezing media with 10% DMSO. Frozen stock was thawed and grown to collect cells for the imaging experiment

   December 3rd, 2023 at 11:47pm

Details

aliases 
  1. fowler-lab:if_f121-6_NPC_bcc
contributing_labs 
  1. Xinxian Deng, UW
  2. Christine Disteche, UW
  3. William Noble, UW
culture_duration 
7
culture_harvest_date 
2021-05-18
culture_start_date 
2021-05-11
differentiation_state 
Nestin positive NPCs derived from Day 11 embryoid bodies followed by single-cell cloning
doubling_number 
14
doubling_time 
~0.5 day
in_vitro_differentiated 
Yes
passage_number 
2
protocols_additional 
  1. 4DN_F123_SOP_170425.pdf
  2. Mouse NPC differentiation protocol.pdf
public_release 
May 17th, 2024
tissue 
neuronal stem cell