ExperimentMic multiplexed FISH on primary motor cortex

multiplexed FISH4DNEXDSE1987
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Genome-wide chromatin imaging by DNA-MERFISH of 2000 loci in mouse primary motor cortex of Mecp2 heterozygous knock-out mice, together with RNA-MERFISH of 240 gene transcripts, Biological replicate 2, Technical replicate 1

   November 15th, 2023 at 9:30pm
Source Publication
Cell type-specific 3D-genome organization and transcription regulation in the brain.
Liu S, Wang CY, et al., Science advances 2025

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSBPM6HI7
Biosample Biosource 
primary motor cortex - 4DNSRSHTV6AX
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
Harvard-Zhuang_lab_CT-STORM_Nikon_Eclipse_Ti-U_Liu-2023_Scope1
Channel
Imaging Rounds
Path
 
ch02
13-97
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Cy3-labeled imaging oligos)
ch03
13-97
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch04
13-97
2000 loci (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)
ch01
0
540/560nm fiducial beads
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