ExperimentSetReplicate

ExperimentSet4DNESYXWQD8L
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Chromatin tracing (ORCA) of HoxA locus in TC1 mouse ESCs, with Pc factors EED and RING1b endogenously tagged with the dTAG inducible degrons, treated with dTAG-13 for 8 hours

   January 24th, 2023 at 12:49pm
Source Publication
Polycomb repression of Hox genes involves spatial feedback but not domain compaction or phase transition.
Murphy SE, Boettiger AN, Nature genetics 2024

Microscope configuration  

The experiments associated with this study are derived from either 33ea326f-a557-4457-becf-55bc860c4bdf or 4fc82c6a-a9cd-43e1-aee8-36db3174b409 microscopes

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
TC1 with EED-dTAG, dTAG-Ring1b
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
Chemical
Assay Details 
Target: 52 consecutive 5-kb regions at HoxA locus (mouse)
Channel
Imaging Rounds
Path
 
ch00
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
1-52
52 consecutive 5-kb regions at HoxA locus (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)

4 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX6OO8EKV
Bio Rep 1, Tec Rep 1
multiplexed FISH on TC1 with EED-dTAG, dTAG-Ring1b
File
4DNFI8OK86NF
FOF-CT - DNA-spot/trace core (csv)
1.98 MB
File
4DNFI9B5OLHF
FOF-CT - spot quality (csv)
3.18 MB
Experiment
4DNEX6COY1F2
Bio Rep 2, Tec Rep 1
multiplexed FISH on TC1 with EED-dTAG, dTAG-Ring1b
File
4DNFIYZDTHMG
FOF-CT - DNA-spot/trace core (csv)
1.95 MB
File
4DNFI8943SZ8
FOF-CT - spot quality (csv)
3.48 MB