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Lab
Dataset
Sample Type
Sample Category
Sample
Organ/System
Tissue Source
Publication
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8 Common Properties
 Experiment Category
Microscopy
 Experiment Type
multiplexed FISH
 Organism
Mouse
 Sample Tier
Unclassified
 Treatments
No value
 Set Type
Replicate
 Status
Released
 Commendations
No value
8 Results
Title
Experiment Type
Biosample
Dataset
Condition
Assay Details
Lab
multiplexed FISH
cerebral cortex
Chromatin tracing in mouse brain
Mouse cerebral cortex
Target
2460 loci (mouse), 1200 loci from 20 regions at 25-kb resolution (mouse), 78 RNAs (mouse), H3K27me2 (mouse), H4K20me3 (mouse), Sf3a2 mouse protein, H3K27me3 (mouse), H3K4me2 (mouse), H3K9me3 (mouse), Mecp2 mouse protein, mH2A.1 (mouse), LINE1 (mouse), Minor Satellite (mouse), Telomeres (mouse), SINEB1 (mouse), Major Satellite (mouse), Chromosomes
multiplexed FISH
ES-E14TG2a.4
Chromatin tracing in mESCs
Mouse ES cells
Target
2460 loci (mouse), 1200 loci from 20 regions at 25-kb resolution (mouse), 70 RNAs (mouse), Fibrillarin (mouse), H3K27ac (mouse), H3K9ac (mouse), H3K9me2 (mouse), H3K9me3 (mouse), H3S10p (mouse), H4K20me1 (mouse), H4K20me2 (mouse), mH2A.1 (mouse), RNA PolII S5p (mouse), Geminin (mouse), H3K27me2 (mouse), H3K27me3 (mouse), H4K16ac (mouse), H4K20me3 (mouse), Lmnb1 mouse protein, Sf3a2 mouse protein, LINE1 (mouse), Minor Satellite (mouse), Telomeres (mouse), SINEB1 (mouse), Chromosomes
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site downstream of Sox2 SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 CTCF sites inserted downstream of both Sox2 and SE with Sox2 RNA FISH
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes, Sox2 mouse transcript, GFP transcript, mCherry transcript
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 CTCF sites inserted between Sox2 and SE with Sox2 RNA FISH
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes, Sox2 mouse transcript, GFP transcript, mCherry transcript
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 mutant CTCF sites inserted between Sox2 and SE
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site downstream of Sox2 SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 CTCF sites inserted downstream of both Sox2 and SE
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 CTCF sites inserted between Sox2 and SE
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes
multiplexed FISH
liver
Chromatin tracing in mammalian tissue
Fetal mouse liver
Target
19 consecutive 5-kb loci on chr19 (mouse), 50 TADs on chr19 (mouse), 55 RNAs from fetal liver marker genes and 82 RNAs from genes on chr19 (mouse), Chromosomes, Cell membrane, Nucleolus
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