Finn et al systematically probed the heterogeneity in genome organization in human fibroblasts by combining high-resolution Hi-C datasets and high-throughput genome imaging. High-throughput Imaging Position MAPing (HIPMap) based on high-throughput fluorescence in situ hybridization (hiFISH) was used to systematically determine the spatial position and distances between combinations of genomic interaction pairs identified by Hi-C.

Dilution Hi-C experiments were performed with HFF-hTERT cells, and various loci were chosen on chromosomes 1, 17 and 18 to test distances of different pairs of loci with high-throughput DNA-FISH (HIPMap). Interaction of TADs was also probed on chromosome 4. Here you can find a summary of the experiments performed.

Microcopy Dataset	Aim	Target List
DNA-FISH on Chr1	Testing different Hi-C frequencies	Targets on Chr1
DNA-FISH on Chr17	Testing different Hi-C frequencies	Targets on Chr17
DNA-FISH on Chr18	Testing different Hi-C frequencies	Targets on Chr18
DNA-FISH on Chr1 and 17	Testing different Hi-C frequencies	-
DNA-FISH on Chr4	Testing TAD interaction	Targets on Chr4