replaced
October 28th, 2019 at 3:14pm
Note: Replaced Biorxiv
Overview
Abstract
Chromosomes are folded so that active and inactive chromatin domains are spatially segregated. Compartmentalization is thought to occur through polymer phase/microphase separation mediated by interactions between loci of similar type. The nature and dynamics of these interactions are not known. We developed liquid chromatin Hi-C to map the stability of associations between loci. Before fixation and Hi-C, chromosomes are fragmented removing the strong polymeric constraint to enable detection of intrinsic locus-locus interaction stabilities. Compartmentalization is stable when fragments are over 10-25 kb. Fragmenting chromatin into pieces smaller than 6 kb leads to gradual loss of genome organization. Dissolution kinetics of chromatin interactions vary for different chromatin domains. Lamin-associated domains are most stable, while interactions among speckle and polycomb-associated loci are more dynamic. Cohesin-mediated loops dissolve after fragmentation, possibly because cohesin rings slide off nearby DNA ends. Liquid chromatin Hi-C provides a genome-wide view of chromosome interaction dynamics. Highlights
Authors
Houda Belaghzal • Tyler Borrman • Andrew D. Stephens • Denis L. Lafontaine • Sergey V. Venev • Zhiping Weng • John F. Marko • Job Dekker
Link
Journal
bioRxiv
doi:10.1101/704957
Published
July 16th, 2019