{"lab": {"status": "current", "title": "Jennifer Cremins, UPENN", "uuid": "b18699f9-e3e9-44e2-8070-d5b044efc09e", "@id": "/labs/jennifer-cremins-lab/", "display_title": "Jennifer Cremins, UPENN", "@type": ["Lab", "Item"], "correspondence": [{"contact_email": "amNyZW1pbnNAc2Vhcy51cGVubi5lZHU=", "@id": "/users/8773d50c-1716-4153-9a18-d0f5ff2aa5ee/", "display_title": "Jennifer Phillips-Cremins"}], "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.b18699f9-e3e9-44e2-8070-d5b044efc09e"]}}, "award": {"uuid": "b3046311-087c-4141-8e98-a099b96ac6cd", "center_title": "Phillips-Cremins", "display_title": "ENGINEERING AND IMAGING 3D GENOME STRUCTURE-FUNCTION DYNAMICS ACROSS TIME SCALES", "name": "1U01DK127405-01", "status": "current", "@type": ["Award", "Item"], "@id": "/awards/1U01DK127405-01/", "description": "RT-CDF:The mammalian genome folds into tens of thousands of long-range looping interactions. A critical unknown is whether and how chromatin loops control gene expression, and a major unresolved question is how the temporal progression of loops relates to transcription dynamics. One major barrier to answering this question is that loops change on a range of timescales, necessitating the use of tools and model systems amenable to tracking and engineering loops longitudinally and in real time on both short and long timing. Here, we propose to develop and apply new engineering and imaging tools to measure, induce, and perturb loops with precise temporal control in three different biological systems spanning minutes, hours, and weeks. At the shortest timescale (minutes, Aim 1), we will examine loop dynamics in human induced pluripotent stem cell-derived neurons in response to electrical stimulation, revealing how interaction frequency is functionally connected to transcriptional bursting of immediate early and secondary response genes. On the timescale of hours (Aim 3), we will elucidate how the architectural protein YY1 connects enhancer-promoter loops that re-assemble upon the exit from mitosis by erythroid cells. On the timescale of weeks (Aim 2), we will use a cellular \u201cTime Machine\u201d to longitudinally track the rare cells that undergo cellular reprogramming, allowing us to dissect the functionality of loop formation and dissolution with single-cell and subcellular resolution during the reprogramming of somatic cells to pluripotency and transition of melanoma cancer cells to a resistant phenotype. Our team consists of a highly productive and collaborative set of junior and senior investigators with complementary expertise and overlapping interests, including Dr. Gerd Blobel (epigenetics, mitosis, loop engineering), Dr. Eric Joyce (Oligopaints imaging), Dr. Bomyi Lim (nascent transcript live cell imaging), Dr. Jennifer Phillips-Cremins (chromatin architecture, loop engineering, neurobiology), Dr. Stanley Qi (CRISPR genome engineering, live cell imaging), and Dr. Arjun Raj (single cell genomics, RNA imaging, reprogramming). We will develop and apply live and fixed cell imaging techniques for chromatin contacts, and in the same cells image nascent transcription. We will build a cadre of synthetic architectural proteins to engineer loops in a time-dependent inducible manner. Successful application of our engineering and imaging tools across biological systems will yield a comprehensive and rigorous assessment of the cause-and-effect relationship between loops and distinct biological phenotypes across timescales.", "project": "4DN", "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "md5sum": "d1f0318f8c64ced1b8315234f10a6eaf", "status": "released", "aliases": ["cremins-lab:5C--ips-80kb-del-EL-boundary_2G5_rep1_qnorm_matrix"], "filename": "4000_binned_6000_smoothed_Proj25-EPIFXS-exp6-5C-2G580kb-ips-rep1-LZ3C1WG5C1-S1_S4_tohg38.csv", "accession": "4DNFIXGLJ6J7", "file_size": 57682132, "file_type": "normalized contact matrix", "description": "ips 80kb cutout downstream quantile normalized matrix at 4kb resolution (quantile normalized from five raw matrices - two WT, two 30kb cutout, and one 80kb cutout)", "file_format": {"file_format": "csv", "status": "released", "display_title": "csv", "@id": "/file-formats/csv/", "uuid": "d13d06cf-218e-4f61-55f0-94f336118b2c", "@type": ["FileFormat", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "date_created": "2021-01-19T20:13:41.079636+00:00", "notes_to_tsv": ["This file contains processed results performed outside of the 4DN-DCIC standardized pipelines. The file and the information about its provenance, i.e. which files were used as input to generate this output was provided by or done in collaboration with the lab that did the experiments to generate the raw data. For more information about the specific analysis performed, please contact the submitting lab or refer to the relevant publication if available."], "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2021-12-16T07:00:53.897843+00:00"}, "produced_from": ["cremins-lab:5C--ips-80kb-del-EL-boundary_2G5_rep1_raw_matrix_hg38"], "content_md5sum": "d1f0318f8c64ced1b8315234f10a6eaf", "public_release": "2021-02-02", "schema_version": "3", "genome_assembly": "GRCh38", "project_release": "2021-02-02", "file_classification": "processed file", "@id": "/files-processed/4DNFIXGLJ6J7/", "@type": ["FileProcessed", "File", "Item"], "uuid": "bfd2cae4-67e1-4b91-b39e-50e2e54cdc39", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "4DNFIXGLJ6J7.csv", "external_references": [], "experiments": [{"status": "released", "accession": "4DNEXJP6CXYD", "display_title": "Capture Hi-C on 7889SA3.5 with HindIII - 4DNEXJP6CXYD", "@type": ["ExperimentCaptureC", "Experiment", "Item"], "uuid": "251a4dc1-90f0-4551-9452-f4607624c231", "@id": "/experiments-capture-c/4DNEXJP6CXYD/", "biosample": {"uuid": "f5afdd74-0c38-4a80-9533-dc9899532e0a", "treatments_summary": "None", "biosource_summary": "7889SA3.5", "@id": "/biosamples/4DNBSGDOOBM2/", "modifications_summary": "Crispr deletion for 80KB IDS deletion", "display_title": "4DNBSGDOOBM2", "@type": ["Biosample", "Item"], "accession": "4DNBSGDOOBM2", "status": "released", "cell_culture_details": [{"in_vitro_differentiated": "No", "@type": ["BiosampleCellCulture", "Item"], "display_title": "BiosampleCellCulture from 2021-01-19", "status": "released", "@id": "/biosample-cell-cultures/134e092a-03d1-440e-91b1-c9819e5fc5fc/", "uuid": "134e092a-03d1-440e-91b1-c9819e5fc5fc", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "biosource": [{"cell_line_tier": "Unclassified", "status": "released", "override_biosource_name": "7889SA3.5", "@id": "/biosources/4DNSRIOTVJ4X/", "biosource_type": "induced pluripotent stem cell", "display_title": "7889SA3.5 - 4DNSRIOTVJ4X", "uuid": "85b1e0d9-6d5a-4ddf-b964-3f712e7949d6", "@type": ["Biosource", "Item"], "tissue": {"status": "released", "@type": ["OntologyTerm", "Item"], "preferred_name": "stem cell", "uuid": "082f5be3-3617-4d3e-9ee9-78df53a71802", "display_title": "stem cell", "term_id": "CL:0000034", "term_name": "stem cell", "@id": "/ontology-terms/CL:0000034/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "cell_line": {"@type": ["OntologyTerm", "Item"], "display_title": "pluripotent stem cell", "uuid": "8816a6d9-1295-4590-88fa-3dff7a2d117b", "preferred_name": "pluripotent stem cell", "status": "released", "term_name": "pluripotent stem cell", "term_id": "CL:0002248", "@id": "/ontology-terms/CL:0002248/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "individual": {"status": "released", "uuid": "bb407e38-3c7f-4782-9099-ed213854859f", "protected_data": false, "@type": ["IndividualHuman", "Individual", "Item"], "display_title": "4DNINF2U62Q5", "@id": "/individuals-human/4DNINF2U62Q5/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "organism": {"display_title": "H. sapiens", "scientific_name": "Homo sapiens", "uuid": "7745b647-ff15-4ff3-9ced-b897d4e2983c", "status": "released", "@id": "/organisms/9606/", "@type": ["Organism", "Item"], "name": "human", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "experiment_type": {"status": "released", "@type": ["ExperimentType", "Item"], "title": "Capture Hi-C", "@id": "/experiment-types/capture-hi-c/", "display_title": "Capture Hi-C", "uuid": "62ddb15e-4907-4b8a-bfca-cdd4371cb179", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "experiment_sets": [{"status": "released", "experimentset_type": "replicate", "uuid": "38302016-deba-4b2b-918e-9313d736c66d", "@id": "/experiment-set-replicates/4DNES39F1QWU/", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "accession": "4DNES39F1QWU", "display_title": "4DNES39F1QWU", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "digestion_enzyme": {"status": "released", "uuid": "2ace2f00-c913-4bac-a6ef-0b316bc2108e", "name": "HindIII", "display_title": "HindIII", "@id": "/enzymes/HindIII/", "@type": ["Enzyme", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "last_modified": {"date_modified": "2023-10-04T20:11:06.147453+00:00"}}], "file_type_detailed": "normalized contact matrix (csv)", "track_and_facet_info": {"experimental_lab": "Jennifer Cremins, UPENN", "experiment_type": "Capture Hi-C", "experiment_bucket": "Derived data files provided by the Cremins Lab", "assay_info": "6MB 5C enriched region", "dataset": "5C on 6Mb of chrX", "condition": "IZ boundary deletion", "replicate_info": "Biorep 1, Techrep 1", "biosource_name": "7889SA3.5", "lab_name": "Jennifer Cremins, UPENN"}, "title": "4DNFIXGLJ6J7", "href": "/files-processed/4DNFIXGLJ6J7/@@download/4DNFIXGLJ6J7.csv", "upload_key": "bfd2cae4-67e1-4b91-b39e-50e2e54cdc39/4DNFIXGLJ6J7.csv", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/bfd2cae4-67e1-4b91-b39e-50e2e54cdc39/4DNFIXGLJ6J7.csv", "tsv_notes": "This file contains processed results performed outside of the 4DN-DCIC standardized pipelines. The file and the information about its provenance, i.e. which files were used as input to generate this output was provided by or done in collaboration with the lab that did the experiments to generate the raw data. For more information about the specific analysis performed, please contact the submitting lab or refer to the relevant publication if available.", "workflow_run_inputs": [{"uuid": "ec3731d3-4774-4135-b730-dcef3bcda2a3", "@id": "/workflow-runs-awsem/ec3731d3-4774-4135-b730-dcef3bcda2a3/", "display_title": "md5 0.2.6 run 2021-01-19 20:15:06.382064", "@type": ["WorkflowRunAwsem", "WorkflowRun", "Item"], "status": "released", "input_files": [{"workflow_argument_name": "input_file", "value": {"uuid": "bfd2cae4-67e1-4b91-b39e-50e2e54cdc39", "filename": "4000_binned_6000_smoothed_Proj25-EPIFXS-exp6-5C-2G580kb-ips-rep1-LZ3C1WG5C1-S1_S4_tohg38.csv", "display_title": "4DNFIXGLJ6J7.csv", "accession": "4DNFIXGLJ6J7", "@type": ["FileProcessed", "File", "Item"], "status": "released", "@id": "/files-processed/4DNFIXGLJ6J7/", "file_format": {"status": "released", "@type": ["FileFormat", "Item"], "@id": "/file-formats/csv/", "display_title": "csv", "uuid": "d13d06cf-218e-4f61-55f0-94f336118b2c", "file_format": "csv", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "output_files": [{"workflow_argument_name": "report"}], "workflow": {"uuid": "c77a117b-9a58-477e-aaa5-291a109a99f6", "@id": "/workflows/4DNWF2HFF82B/", "display_title": "md5 0.2.6 - 4DNWF2HFF82B", "title": "md5 0.2.6", "status": "released", "@type": ["Workflow", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"uuid": "e938a5ac-e0c2-4283-9382-7671862fd328", "@id": "/workflow-runs-awsem/e938a5ac-e0c2-4283-9382-7671862fd328/", "display_title": "File Provenance Tracking Workflow run on 2021-02-04 16:03:20.110165", "@type": ["WorkflowRunAwsem", "WorkflowRun", "Item"], "status": "released", "input_files": [{"workflow_argument_name": "inputs", "value": {"uuid": "bfd2cae4-67e1-4b91-b39e-50e2e54cdc39", "filename": "4000_binned_6000_smoothed_Proj25-EPIFXS-exp6-5C-2G580kb-ips-rep1-LZ3C1WG5C1-S1_S4_tohg38.csv", "display_title": "4DNFIXGLJ6J7.csv", "accession": "4DNFIXGLJ6J7", "@type": ["FileProcessed", "File", "Item"], "status": "released", "@id": "/files-processed/4DNFIXGLJ6J7/", "file_format": {"status": "released", "@type": ["FileFormat", "Item"], "@id": "/file-formats/csv/", "display_title": "csv", "uuid": "d13d06cf-218e-4f61-55f0-94f336118b2c", "file_format": "csv", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "output_files": [{"workflow_argument_name": "outputs", "value": {"status": "released", "uuid": "d95ca16c-52b6-4d2f-abf6-387ab1cd8c69", "display_title": "4DNFIA8P94BX.csv", "@id": "/files-processed/4DNFIA8P94BX/", "accession": "4DNFIA8P94BX", "@type": ["FileProcessed", "File", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "file_format": {"file_format": "csv", "@type": ["FileFormat", "Item"], "@id": "/file-formats/csv/", "display_title": "csv", "status": "released", "uuid": "d13d06cf-218e-4f61-55f0-94f336118b2c", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}}], "workflow": {"uuid": "bef50397-4d72-4ed1-9c78-100e14e5c47f", "@id": "/workflows/4DNWFBFZYHIE/", "display_title": "File Provenance Tracking Workflow - 4DNWFBFZYHIE", "title": "File Provenance Tracking Workflow", "status": "released", "@type": ["Workflow", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "workflow_run_outputs": [{"display_title": "File Provenance Tracking Workflow run on 2021-02-04 15:10:27.686535", "@type": ["WorkflowRunAwsem", "WorkflowRun", "Item"], "status": "released", "@id": "/workflow-runs-awsem/ede0fa29-f968-4120-89b1-a8d15c012731/", "uuid": "ede0fa29-f968-4120-89b1-a8d15c012731", "input_files": [{"workflow_argument_name": "inputs", "value": {"accession": "4DNFIA7JEN1Z", "@type": ["FileProcessed", "File", "Item"], "filename": "4000_binned_Proj25-EPIFXS-exp6-5C-2G580kb-ips-rep1-LZ3C1WG5C1-S1_S4_hg38v2.csv", "uuid": "9e6857dc-5434-4ee4-ba01-1d811f72c951", "status": "released", "@id": "/files-processed/4DNFIA7JEN1Z/", "display_title": "4DNFIA7JEN1Z.csv", "file_format": {"@type": ["FileFormat", "Item"], "@id": "/file-formats/csv/", "uuid": "d13d06cf-218e-4f61-55f0-94f336118b2c", "status": "released", "file_format": "csv", "display_title": "csv", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "output_files": [{"workflow_argument_name": "outputs", "value": {"accession": "4DNFIXGLJ6J7", "@type": ["FileProcessed", "File", "Item"], "display_title": "4DNFIXGLJ6J7.csv", "status": "released", "@id": "/files-processed/4DNFIXGLJ6J7/", "uuid": "bfd2cae4-67e1-4b91-b39e-50e2e54cdc39", "file_format": {"status": "released", "uuid": "d13d06cf-218e-4f61-55f0-94f336118b2c", "@id": "/file-formats/csv/", "file_format": "csv", "display_title": "csv", "@type": ["FileFormat", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "workflow": {"status": "released", "@id": "/workflows/4DNWFBFZYHIE/", "title": "File Provenance Tracking Workflow", "@type": ["Workflow", "Item"], "display_title": "File Provenance Tracking Workflow - 4DNWFBFZYHIE", "uuid": "bef50397-4d72-4ed1-9c78-100e14e5c47f", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "experiment_sets": [], "source_experiment_sets": [{"@id": "/experiment-set-replicates/4DNES39F1QWU/", "status": "released", "uuid": "38302016-deba-4b2b-918e-9313d736c66d", "display_title": "4DNES39F1QWU", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "@context": "/terms/", "aggregated-items": {"last_modified": [{"parent": "/experiments-capture-c/4DNEXJP6CXYD/", "embedded_path": "experiments.last_modified", "item": {"date_modified": "2023-10-04T20:11:06.147453+00:00"}}, {"parent": "/files-processed/4DNFIXGLJ6J7/", "embedded_path": "last_modified", "item": {"date_modified": "2021-12-16T07:00:53.897843+00:00"}}]}, "validation-errors": []}