{"lab": {"uuid": "cf5abe29-1cf9-4413-b02e-664b47a6d53d", "title": "Ana Pombo, MDC", "correspondence": [{"contact_email": "YW5hLnBvbWJvQG1kYy1iZXJsaW4uZGU=", "@id": "/users/bce4f2b5-9a2a-4db2-9dd6-7d61f19025c1/", "display_title": "Ana Pombo"}], "@id": "/labs/ana-pombo-lab/", "status": "current", "display_title": "Ana Pombo, MDC", "@type": ["Lab", "Item"], "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.cf5abe29-1cf9-4413-b02e-664b47a6d53d"]}}, "award": {"uuid": "ee89349a-1d43-4048-9142-bc750f0f3b32", "@type": ["Award", "Item"], "center_title": "CDIMV - Ren", "description": "CDIMV: The transcriptional regulatory sequences communicate with each other dynamically in the 3D nuclear space to direct cell type specific gene expression. Currently, a major barrier to understanding the transcriptional regulatory programs is the lack of tools, models and maps to explore the chromatin architecture in diverse cell types and physiological contexts. We will address this pressing need by deploying transformative technologies to study the chromatin architecture in mammalian cells at an unprecedented resolution and scale. Specifically, we will generate navigable, cell-type-specific reference maps of chromatin architecture in the mouse, macaque and human brains by integrating high resolution and high throughput imaging and orthogonal single-cell-based genomic methods. We will also dissect the role of chromatin architecture in gene regulation through a set of controlled perturbation experiments in the mouse ES cells (ESC) and ESC-derived neural progenitor cells (NPC). We will develop structural models of chromatin organization with advanced polymer physics and statistical learning methods, and validate their predictive power in embryonic stem cells and in ex vivo brain slices. Finally, we will make the reference maps, analytical tools, visualization methods and structural models available to the broader community. The proposed research project will dramatically transform our ability to analyze the 4D Nucleome of complex tissues, and produce the much-needed maps, tools and models for understanding the gene regulatory programs encoded in the linear genome sequences.", "@id": "/awards/1UM1HG011585-01/", "status": "current", "project": "4DN", "name": "1UM1HG011585-01", "display_title": "CENTER FOR INTEGRATED MULTI-MODAL AND MULTI-SCALE NUCLEOME RESEARCH", "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "md5sum": "b26cd2bd65299f4817f251d6041831c0", "status": "released", "aliases": ["ana-pombo-lab:GAM-on-H1hESC-Tier-1-NEW-R1R2-merged-segregation-table-at100Kb-NPMI-normalized-pairwise-contact-matrices-at25Kb-mcool"], "filename": "GAM_H1hESC_NEW_R1R2_merged.NPMI.normalized.contact.matrices.at100Kb.mcool", "accession": "4DNFI53A5Z59", "file_size": 2846770353, "file_type": "normalized contact matrix", "description": "GAM H1hESC NEW R1R2 merged NPMI normalized pair-wise chromatin contact matrices at100Kb mcool format", "file_format": {"file_format": "mcool", "@id": "/file-formats/mcool/", "uuid": "d13d06cf-218e-4f61-ccf0-91f226248b2c", "status": "released", "display_title": "mcool", "@type": ["FileFormat", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "higlass_uid": "4afd8e78-8280-4844-8d34-7ba8e3a9d3f2", "date_created": "2024-03-13T11:48:38.689854+00:00", "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2025-09-05T16:46:44.584367+00:00"}, "content_md5sum": "b26cd2bd65299f4817f251d6041831c0", "public_release": "2025-08-15", "quality_metric": {"@type": ["QualityMetricMcool", "QualityMetric", "Item"], "uuid": "09e34743-37f1-49c9-aaf3-ac170ea41385", "display_title": "QualityMetricMcool from 2024-03-13", "status": "released", "@id": "/quality-metrics-mcool/09e34743-37f1-49c9-aaf3-ac170ea41385/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "quality_metric_summary": [{"title": "Failed Balancing", "value": "None", "tooltip": "Resolutions where balancing failed", "numberType": "string"}]}, "schema_version": "3", "static_content": [{"content": {"@id": "/higlass-view-configs/88a02603-356c-47b0-895b-6ade2cf435eb/", "award": {"status": "current", "@type": ["Award", "Item"], "uuid": "ee89349a-1d43-4048-9142-bc750f0f3b32", "@id": "/awards/1UM1HG011585-01/", "display_title": "CENTER FOR INTEGRATED MULTI-MODAL AND MULTI-SCALE NUCLEOME RESEARCH", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "description": "", "@type": ["HiglassViewConfig", "UserContent", "Item"], "uuid": "88a02603-356c-47b0-895b-6ade2cf435eb", "lab": {"status": "current", "display_title": "Ana Pombo, MDC", "uuid": "cf5abe29-1cf9-4413-b02e-664b47a6d53d", "@type": ["Lab", "Item"], "@id": "/labs/ana-pombo-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.cf5abe29-1cf9-4413-b02e-664b47a6d53d"]}}, "name": "88a02603-356c-47b0-895b-6ade2cf435eb", "contributing_labs": [], "display_title": "4DNFI53A5Z59 - normalized contact matrix for H1-hESC (Tier 1) GAM", "title": "4DNFI53A5Z59 - normalized contact matrix for H1-hESC (Tier 1) GAM", "filetype": "HiglassViewConfig", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}, "location": "tab:higlass", "description": "auto_generated_higlass_view_config"}], "genome_assembly": "GRCh38", "project_release": "2024-03-19", "file_classification": "processed file", "@id": "/files-processed/4DNFI53A5Z59/", "@type": ["FileProcessed", "File", "Item"], "uuid": "e5230982-53e1-44e2-a319-5225b4578cfb", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "4DNFI53A5Z59.mcool", "external_references": [], "experiments": [], "file_type_detailed": "normalized contact matrix (mcool)", "track_and_facet_info": {"dataset": "GAM on H1-ESC cells", "condition": "updated WGA protocol", "experimental_lab": "Ana Pombo, MDC", "replicate_info": "merged replicates", "experiment_bucket": "Pombo Lab - GAM pipeline output 100 Kb bin", "experiment_type": "GAM", "biosource_name": "H1-hESC (Tier 1)", "lab_name": "Ana Pombo, MDC", "track_title": "normalized contact matrix for H1-hESC (Tier 1) GAM"}, "title": "4DNFI53A5Z59", "href": "/files-processed/4DNFI53A5Z59/@@download/4DNFI53A5Z59.mcool", "upload_key": "e5230982-53e1-44e2-a319-5225b4578cfb/4DNFI53A5Z59.mcool", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/e5230982-53e1-44e2-a319-5225b4578cfb/4DNFI53A5Z59.mcool", "tsv_notes": "", "workflow_run_inputs": [{"status": "released", "display_title": "md5 0.2.6 run 2024-03-13 13:26:59.266072", "@id": "/workflow-runs-awsem/a93c7c90-857f-4770-8b2d-200451880af1/", "uuid": "a93c7c90-857f-4770-8b2d-200451880af1", "@type": ["WorkflowRunAwsem", "WorkflowRun", "Item"], "output_files": [{"workflow_argument_name": "report"}], "workflow": {"display_title": "md5 0.2.6 - 4DNWF2HFF82B", "title": "md5 0.2.6", "status": "released", "@id": "/workflows/4DNWF2HFF82B/", "@type": ["Workflow", "Item"], "uuid": "c77a117b-9a58-477e-aaa5-291a109a99f6", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "input_files": [{"workflow_argument_name": "input_file", "value": {"display_title": "4DNFI53A5Z59.mcool", "uuid": "e5230982-53e1-44e2-a319-5225b4578cfb", "status": "released", "filename": "GAM_H1hESC_NEW_R1R2_merged.NPMI.normalized.contact.matrices.at100Kb.mcool", "@id": "/files-processed/4DNFI53A5Z59/", "@type": ["FileProcessed", "File", "Item"], "accession": "4DNFI53A5Z59", "file_format": {"status": "released", "display_title": "mcool", "file_format": "mcool", "@type": ["FileFormat", "Item"], "uuid": "d13d06cf-218e-4f61-ccf0-91f226248b2c", "@id": "/file-formats/mcool/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"status": "released", "display_title": "mcoolQC v1 run 2024-03-13 13:46:21.895885", "@id": "/workflow-runs-awsem/53515402-8048-4684-aca7-189f488acc10/", "uuid": "53515402-8048-4684-aca7-189f488acc10", "@type": ["WorkflowRunAwsem", "WorkflowRun", "Item"], "output_files": [{"workflow_argument_name": "mcool_qc", "value_qc": {"@id": "/quality-metrics-mcool/09e34743-37f1-49c9-aaf3-ac170ea41385/", "overall_quality_status": "FAIL", "status": "released", "display_title": "QualityMetricMcool from 2024-03-13", "@type": ["QualityMetricMcool", "QualityMetric", "Item"], "uuid": "09e34743-37f1-49c9-aaf3-ac170ea41385", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "workflow": {"display_title": "Quality metrics for mcool files - 4DNWFXKEC8OX", "title": "Quality metrics for mcool files", "status": "released", "@id": "/workflows/4DNWFXKEC8OX/", "@type": ["Workflow", "Item"], "uuid": "0bf9f47a-dec1-4324-9b41-fa183880a7db", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "input_files": [{"workflow_argument_name": "mcoolfile", "value": {"display_title": "4DNFI53A5Z59.mcool", "uuid": "e5230982-53e1-44e2-a319-5225b4578cfb", "status": "released", "filename": "GAM_H1hESC_NEW_R1R2_merged.NPMI.normalized.contact.matrices.at100Kb.mcool", "@id": "/files-processed/4DNFI53A5Z59/", "@type": ["FileProcessed", "File", "Item"], "accession": "4DNFI53A5Z59", "file_format": {"status": "released", "display_title": "mcool", "file_format": "mcool", "@type": ["FileFormat", "Item"], "uuid": "d13d06cf-218e-4f61-ccf0-91f226248b2c", "@id": "/file-formats/mcool/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "workflow_run_outputs": [], "experiment_sets": [{"@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "uuid": "b0cb9915-1a6f-4b2d-814c-61364fdd1680", "accession": "4DNESVAMUDHA", "status": "released", "@id": "/experiment-set-replicates/4DNESVAMUDHA/", "display_title": "4DNESVAMUDHA", "last_modified": {"date_modified": "2025-08-15T19:32:31.640636+00:00"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "source_experiment_sets": [{"@id": "/experiment-set-replicates/4DNESVAMUDHA/", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "display_title": "4DNESVAMUDHA", "uuid": "b0cb9915-1a6f-4b2d-814c-61364fdd1680", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "@context": "/terms/", "aggregated-items": {"last_modified": [{"parent": "/experiment-set-replicates/4DNESVAMUDHA/", "embedded_path": "experiment_sets.last_modified", "item": {"date_modified": "2025-08-15T19:32:31.640636+00:00"}}, {"parent": "/files-processed/4DNFI53A5Z59/", "embedded_path": "last_modified", "item": {"date_modified": "2025-09-05T16:46:44.584367+00:00"}}]}, "validation-errors": []}