![](\"https://s3.amazonaws.com/4dn-dcic-public/static-pages/InfoBoxes/dHiC.png\"/)
\n \n Image source: Lieberman-aiden et al., 2009, Figure 1A\n
This experiment set is replaced by \n4DNESYTUBW2E. \nThe experiment type was initially submitted as \"Dilution Hi-C\" \nbut it was subsequently determined that the correct experiment \ntype is \"in situ Hi-C\". \nIn order to correct this mistake, this Experiment Set and \nassociated Experiment have been replaced with new ones of \nthe correct type. \nThis correction does not affect any file or file processing \npipeline associated with this data set. \nBoth raw and processed files linked to this experiment or the \nassociated items remain unchanged.
Data Use Guidelines: This is a data set generated by the \n4DN Network and made freely available to the scientific \ncommunity. If you are intending to use these data for a \npublication, we ask that you please contact the data \ngenerating lab to discuss possible coordinated publication. \nIn your manuscript, please cite the 4DN White Paper \n(doi:10.1038/nature23884) \nand the 4DN Data Portal paper \n(doi:10.1038/s41467-022-29697-4), \nand please acknowledge the 4DN lab which generated the data. Please direct any questions to the Data Coordination and Integration Center.
\nDilution Hi-C is a method to detect and quantify the pairwise interactions between chromosome regions across the entire genome. It was developed in 2009 and it was the first genome-wide chromosome conformation capture technique. \n
\n\nThe protocol involves cross-linking the cells with formaldehyde to form links between physically adjacent DNA regions. Then, the cells are lysed and a restriction enzyme is used to digest the chromatin into multiple DNA fragments. The resulting fragments are biotinylated by end filling of the fragments ends. The fragments are then ligated in an extremely diluted solution in order to favor the ligation of the cross-linked fragments. Then, the DNA is purified and shreared. The biotinylated fragments are pulled down from the solution with streptavidin beads and the library is constructed and sequenced. Analysis of the resulting paired-end short read sequences produces a matrix that shows the number of interactions between different DNA regions.\n
\n\nAdvances in DNA sequencing depth and molecular techniques led to the development of a improved version of this technique known as in situ Hi-C that provides higher resolution, higher accuracy, and a much faster protocol. \n
\n\nSee Lieberman-aiden et al., 2009 for more details on dilution Hi-C.\n
\n\n \nDilution Hi-C is a method to detect and quantify the pairwise interactions between chromosome regions across the entire genome. It was developed in 2009 and it was the first genome-wide chromosome conformation capture technique. \n
\n\nThe protocol involves cross-linking the cells with formaldehyde to form links between physically adjacent DNA regions. Then, the cells are lysed and a restriction enzyme is used to digest the chromatin into multiple DNA fragments. The resulting fragments are biotinylated by end filling of the fragments ends. The fragments are then ligated in an extremely diluted solution in order to favor the ligation of the cross-linked fragments. Then, the DNA is purified and shreared. The biotinylated fragments are pulled down from the solution with streptavidin beads and the library is constructed and sequenced. Analysis of the resulting paired-end short read sequences produces a matrix that shows the number of interactions between different DNA regions.\n
\n\nAdvances in DNA sequencing depth and molecular techniques led to the development of a improved version of this technique known as in situ Hi-C that provides higher resolution, higher accuracy, and a much faster protocol. \n
\n\nSee Lieberman-aiden et al., 2009 for more details on dilution Hi-C.\n
\n\n \nDilution Hi-C is a method to detect and quantify the pairwise interactions between chromosome regions across the entire genome. It was developed in 2009 and it was the first genome-wide chromosome conformation capture technique. \n
\n\nThe protocol involves cross-linking the cells with formaldehyde to form links between physically adjacent DNA regions. Then, the cells are lysed and a restriction enzyme is used to digest the chromatin into multiple DNA fragments. The resulting fragments are biotinylated by end filling of the fragments ends. The fragments are then ligated in an extremely diluted solution in order to favor the ligation of the cross-linked fragments. Then, the DNA is purified and shreared. The biotinylated fragments are pulled down from the solution with streptavidin beads and the library is constructed and sequenced. Analysis of the resulting paired-end short read sequences produces a matrix that shows the number of interactions between different DNA regions.\n
\n\nAdvances in DNA sequencing depth and molecular techniques led to the development of a improved version of this technique known as in situ Hi-C that provides higher resolution, higher accuracy, and a much faster protocol. \n
\n\nSee Lieberman-aiden et al., 2009 for more details on dilution Hi-C.\n
\n![](\"https://s3.amazonaws.com/4dn-dcic-public/static-pages/InfoBoxes/dHiC.png\")
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