{"lab": {"@type": ["Lab", "Item"], "correspondence": [{"contact_email": "eWlqdW4ucnVhbkBqYXgub3Jn", "@id": "/users/d8ac229e-ec08-4411-be38-dc779520ea62/", "display_title": "Yijun Ruan"}], "status": "current", "display_title": "Yijun Ruan, JAX", "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "@id": "/labs/yijun-ruan-lab/", "title": "Yijun Ruan, JAX", "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}, "award": {"name": "1U54DK107967-01", "@id": "/awards/1U54DK107967-01/", "uuid": "029a2578-43dc-4343-8f41-694518cce304", "center_title": "NOFIC - Ruan", "display_title": "NUCLEOME POSITIONING SYSTEM FOR SPATIOTEMPORAL GENOME ORGANIZATION AND REGULATION", "description": "NOFIC: This proposal seeks to fulfill a community need for a comprehensive, high-resolution genome-mapping platform that will enable investigation of the structural, functional and spatiotemporal organization of the human genome. Our ultimate goal is to deliver complex chromatin interaction network maps in the context of 3D genome structures from which the dynamics of individual genomic elements can be monitored and referenced. Here, we propose to develop a Nucleome Positioning System (NPS)-comprised of 1) a robust genome- wide mapping technology platform, 2) advanced computational modeling algorithms and 3) state-of-the-art nuclear imaging methods-that will allow users community-wide to uncover the regulatory functions of 3D genome organization in human cells. NPS will be based upon the established ChIA-PET method (1,2), enhanced by process optimizations-i.e., microfluidic-based miniaturization and Tn5-transposase-based library preparation-to facilitate the study of chromatin interactions mediated by protein factors across a broader range of human cell types (Aim 1, see also Mapping Technology Development Component). We will also optimize RICh-PET for the comprehensive mapping of chromatin interactions mediated by non-coding RNAs (Aim 1). The high-quality mapping data generated through these optimization efforts will be analyzed by a new computational platform (Three-Dimensional Nucleome Modeling Engine, or 3D-NOME) that makes use of hierarchical multi-scaling to model 3D genome structures (Aim 2, Data Analysis and Modeling component). We will also complement the 3D modeling with transcriptome, epigenome and SNP data associated with genetic diseases (GWAS) to provide functional annotation to structural units (Aim 2). We will continue by developing strategies to validate the nucleome geometry predicted by 3D-NOME both structurally, using new nuclear imaging technologies, and functionally, using cutting-edge genome- and epigenome-editing approaches, in both human cell lines and mouse models (Aim 3, Biological Validation Component). Finally, we will implement NPS to generate pilot 3D genome maps from a wide range of human cell lines and primary immune cells sorted from whole blood, to elucidate the spatiotemporal dynamics of human genome organization over major developmental and hematopoietic cell lineages, as well as among differentiating lymphocytes involved in the immune response (Aim 4, Data Generation Component). Together, these efforts will yield a powerful set of sophisticated, high-quality tools and mapping data for the larger research community, and will help establish the standards for future 3D/4D nucleome studies. They will also provide insights into the broad mechanisms that organize the structure and regulate the function of the human genome, as well as the specific mechanisms by which immune responses are regulated at the nuclear level.", "@type": ["Award", "Item"], "status": "current", "project": "4DN", "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "badges": [{"badge": {"badge_icon": "/static/img/badges/replicates-orange-circle.svg", "@id": "/badges/replicate-numbers/", "@type": ["Badge", "Item"], "uuid": "24a64a84-3c33-4d76-aaf2-e5ef45eff347", "display_title": "Replicate Numbers", "warning": "Replicate Numbers", "badge_classification": "Warning", "title": "Replicate Numbers", "description": "Issues with replicate numbers", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "messages": ["Replicate set contains only a single biological replicate"]}], "status": "released", "aliases": ["ruan-lab:expset_chipseq_HCT116-RAD-mAC_RNAPII_auxin-0h"], "accession": "4DNESKLX2LUW", "condition": "RNAPII antibody - auxin no treatment", "description": "ChIP-seq in HCT116-RAD-mAC using antibody RNAPII treated with auxin-0h", "date_created": "2024-06-28T17:23:26.497286+00:00", "submitted_by": {"error": "no view permissions"}, "dataset_label": "ChIP-seq in HCT116", "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2024-11-20T06:46:14.851348+00:00"}, "public_release": "2024-10-21", "replicate_exps": [{"bio_rep_no": 1, "tec_rep_no": 1, "replicate_exp": {"display_title": "ChIP-seq against RNA Pol II on HCT116 (Tier 2) - 4DNEXACWA3ZI", "accession": "4DNEXACWA3ZI", "@type": ["ExperimentSeq", "Experiment", "Item"], "@id": "/experiments-seq/4DNEXACWA3ZI/", "status": "released", "uuid": "2f451b23-1f86-40ae-9080-9daa14c0d18d", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "schema_version": "2", "static_content": [{"content": {"contributing_labs": [], "award": {"@id": "/awards/1U54DK107967-01/", "status": "current", "display_title": "NUCLEOME POSITIONING SYSTEM FOR SPATIOTEMPORAL GENOME ORGANIZATION AND REGULATION", "@type": ["Award", "Item"], "uuid": "029a2578-43dc-4343-8f41-694518cce304", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "uuid": "0c4ff52a-0a7e-4df6-a1b4-4438fb9e6e88", "title": "4DNESKLX2LUW - Processed files", "@type": ["HiglassViewConfig", "UserContent", "Item"], "name": "0c4ff52a-0a7e-4df6-a1b4-4438fb9e6e88", "@id": "/higlass-view-configs/0c4ff52a-0a7e-4df6-a1b4-4438fb9e6e88/", "filetype": "HiglassViewConfig", "lab": {"status": "current", "@type": ["Lab", "Item"], "display_title": "Yijun Ruan, JAX", "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "@id": "/labs/yijun-ruan-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}, "description": "4DNESKLX2LUW (ChIP-seq in HCT116-RAD-mAC using antibody RNAPII treated with auxin-0h): 4DNFIFNJW3NE, 4DNFIEYILGTZ, 4DNFIOAEB4C4", "display_title": "4DNESKLX2LUW - Processed files", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}, "location": "tab:processed-files", "description": "auto_generated_higlass_view_config"}], "processed_files": [{"md5sum": "dd0d2e2b60f5acd4494e50f589ecb47f", "file_type_detailed": "peaks (bigbed)", "status": "released", "@type": ["FileProcessed", "File", "Item"], "display_title": "4DNFIFNJW3NE.bb", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/57b30cd2-d5f9-4b76-94f0-612a4d520927/4DNFIFNJW3NE.bb", "file_format": {"@type": ["FileFormat", "Item"], "status": "released", "uuid": "bbafb7f8-98d5-48d7-8e75-dc40a5186e19", "display_title": "bigbed", "@id": "/file-formats/bigbed/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "higlass_uid": "8557d63a-7f1d-41ec-a1fd-2d0dca685b71", "upload_key": "57b30cd2-d5f9-4b76-94f0-612a4d520927/4DNFIFNJW3NE.bb", "accession": "4DNFIFNJW3NE", "file_classification": "processed file", "uuid": "57b30cd2-d5f9-4b76-94f0-612a4d520927", "genome_assembly": "GRCh38", "file_size": 958965, "file_type": "peaks", "href": "/files-processed/4DNFIFNJW3NE/@@download/4DNFIFNJW3NE.bb", "@id": "/files-processed/4DNFIFNJW3NE/", "quality_metric": {"display_title": "QualityMetricChipseqV2 from 2024-11-14", "url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFIFNJW3NE/qc_report.html", "status": "released", "uuid": "460d61fb-5d2d-4feb-890a-47438c1e3159", "@type": ["QualityMetricChipseqV2", "QualityMetric", "Item"], "overall_quality_status": "PASS", "@id": "/quality-metrics-chipseq-v2/460d61fb-5d2d-4feb-890a-47438c1e3159/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "static_content": [{"location": "tab:higlass", "description": "auto_generated_higlass_view_config", "content": {"status": "released", "@id": "/higlass-view-configs/3caf0658-7d77-4183-83b4-f29ae6d2f13b/", "display_title": "4DNFIFNJW3NE - peaks for HCT116 (Tier 2) ChIP-seq RNA Pol II", "@type": ["HiglassViewConfig", "UserContent", "Item"], "uuid": "3caf0658-7d77-4183-83b4-f29ae6d2f13b", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "contributing_labs": [{"uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "name": "yijun-ruan-lab", "@type": ["Lab", "Item"], "@id": "/labs/yijun-ruan-lab/", "status": "current", "display_title": "Yijun Ruan, JAX", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}], "lab": {"name": "4dn-dcic-lab", "display_title": "4DN DCIC, HMS", "status": "current", "@type": ["Lab", "Item"], "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@id": "/labs/4dn-dcic-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "external_references": [], "track_and_facet_info": {"dataset": "ChIP-seq in HCT116", "condition": "RNAPII antibody - auxin no treatment", "experimental_lab": "Yijun Ruan, JAX", "replicate_info": "unreplicated", "experiment_bucket": "processed file", "experiment_type": "ChIP-seq", "assay_info": "RNA Pol II", "biosource_name": "HCT116 (Tier 2)", "lab_name": "4DN DCIC, HMS", "track_title": "peaks for HCT116 (Tier 2) ChIP-seq RNA Pol II"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "last_modified": {"date_modified": "2025-01-07T20:48:00.822491+00:00"}}, {"md5sum": "dd0d2e2b60f5acd4494e50f589ecb47f", "file_type_detailed": "conservative peaks (bigbed)", "status": "released", "@type": ["FileProcessed", "File", "Item"], "display_title": "4DNFIEYILGTZ.bb", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/a9e3d5b0-8ef3-473f-9908-b75026b0bfdc/4DNFIEYILGTZ.bb", "file_format": {"@type": ["FileFormat", "Item"], "status": "released", "uuid": "bbafb7f8-98d5-48d7-8e75-dc40a5186e19", "display_title": "bigbed", "@id": "/file-formats/bigbed/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "higlass_uid": "ccea2853-78de-4a47-8769-69e4f335f3fe", "upload_key": "a9e3d5b0-8ef3-473f-9908-b75026b0bfdc/4DNFIEYILGTZ.bb", "accession": "4DNFIEYILGTZ", "file_classification": "processed file", "uuid": "a9e3d5b0-8ef3-473f-9908-b75026b0bfdc", "genome_assembly": "GRCh38", "file_size": 958965, "file_type": "conservative peaks", "href": "/files-processed/4DNFIEYILGTZ/@@download/4DNFIEYILGTZ.bb", "@id": "/files-processed/4DNFIEYILGTZ/", "static_content": [{"location": "tab:higlass", "description": "auto_generated_higlass_view_config", "content": {"status": "released", "@id": "/higlass-view-configs/7fe30413-eae5-423e-a513-8a0c0b36d879/", "display_title": "4DNFIEYILGTZ - conservative peaks for HCT116 (Tier 2) ChIP-seq RNA Pol II", "@type": ["HiglassViewConfig", "UserContent", "Item"], "uuid": "7fe30413-eae5-423e-a513-8a0c0b36d879", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "contributing_labs": [{"uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "name": "yijun-ruan-lab", "@type": ["Lab", "Item"], "@id": "/labs/yijun-ruan-lab/", "status": "current", "display_title": "Yijun Ruan, JAX", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}], "lab": {"name": "4dn-dcic-lab", "display_title": "4DN DCIC, HMS", "status": "current", "@type": ["Lab", "Item"], "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@id": "/labs/4dn-dcic-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "external_references": [], "track_and_facet_info": {"dataset": "ChIP-seq in HCT116", "condition": "RNAPII antibody - auxin no treatment", "experimental_lab": "Yijun Ruan, JAX", "replicate_info": "unreplicated", "experiment_bucket": "processed file", "experiment_type": "ChIP-seq", "assay_info": "RNA Pol II", "biosource_name": "HCT116 (Tier 2)", "lab_name": "4DN DCIC, HMS", "track_title": "conservative peaks for HCT116 (Tier 2) ChIP-seq RNA Pol II"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "last_modified": {"date_modified": "2025-01-07T20:47:59.155257+00:00"}}, {"md5sum": "4b0a28aa631f678f3e86d6dc49d5316a", "file_type_detailed": "signal fold change (bw)", "status": "released", "@type": ["FileProcessed", "File", "Item"], "display_title": "4DNFIOAEB4C4.bw", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/3ec0e9cc-cba2-4ba9-ae27-e3ad279887af/4DNFIOAEB4C4.bw", "file_format": {"@type": ["FileFormat", "Item"], "status": "released", "uuid": "d1311111-218e-4f61-aaf0-91f226248b2c", "display_title": "bw", "@id": "/file-formats/bw/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "higlass_uid": "8eaeb283-81f9-44f6-afd1-7aedf234ba17", "upload_key": "3ec0e9cc-cba2-4ba9-ae27-e3ad279887af/4DNFIOAEB4C4.bw", "accession": "4DNFIOAEB4C4", "file_classification": "processed file", "uuid": "3ec0e9cc-cba2-4ba9-ae27-e3ad279887af", "genome_assembly": "GRCh38", "file_size": 1543963382, "file_type": "signal fold change", "href": "/files-processed/4DNFIOAEB4C4/@@download/4DNFIOAEB4C4.bw", "@id": "/files-processed/4DNFIOAEB4C4/", "static_content": [{"location": "tab:higlass", "description": "auto_generated_higlass_view_config", "content": {"status": "released", "@id": "/higlass-view-configs/5f24b65c-8d0c-4070-89a4-5a8d2f56fccf/", "display_title": "4DNFIOAEB4C4 - signal fold change for HCT116 (Tier 2) ChIP-seq RNA Pol II", "@type": ["HiglassViewConfig", "UserContent", "Item"], "uuid": "5f24b65c-8d0c-4070-89a4-5a8d2f56fccf", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "contributing_labs": [{"uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "name": "yijun-ruan-lab", "@type": ["Lab", "Item"], "@id": "/labs/yijun-ruan-lab/", "status": "current", "display_title": "Yijun Ruan, JAX", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}], "lab": {"name": "4dn-dcic-lab", "display_title": "4DN DCIC, HMS", "status": "current", "@type": ["Lab", "Item"], "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@id": "/labs/4dn-dcic-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "external_references": [], "track_and_facet_info": {"dataset": "ChIP-seq in HCT116", "condition": "RNAPII antibody - auxin no treatment", "experimental_lab": "Yijun Ruan, JAX", "replicate_info": "unreplicated", "experiment_bucket": "processed file", "experiment_type": "ChIP-seq", "assay_info": "RNA Pol II", "biosource_name": "HCT116 (Tier 2)", "lab_name": "4DN DCIC, HMS", "track_title": "signal fold change for HCT116 (Tier 2) ChIP-seq RNA Pol II"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "last_modified": {"date_modified": "2025-01-07T20:47:57.673939+00:00"}}], "project_release": "2024-10-21", "experiments_in_set": [{"@type": ["ExperimentSeq", "Experiment", "Item"], "uuid": "2f451b23-1f86-40ae-9080-9daa14c0d18d", "status": "released", "files": [{"file_size": 2861715062, "@type": ["FileFastq", "File", "Item"], "accession": "4DNFI1DK2FFU", "file_type_detailed": "reads (fastq)", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/files/9ca21ce1-c404-4549-8a49-0d96635952d4/4DNFI1DK2FFU.fastq.gz", "uuid": "9ca21ce1-c404-4549-8a49-0d96635952d4", "@id": "/files-fastq/4DNFI1DK2FFU/", "href": "/files-fastq/4DNFI1DK2FFU/@@download/4DNFI1DK2FFU.fastq.gz", "file_classification": "raw file", "dbxrefs": ["SRA:SRR18530783"], "display_title": "4DNFI1DK2FFU.fastq.gz", "upload_key": "9ca21ce1-c404-4549-8a49-0d96635952d4/4DNFI1DK2FFU.fastq.gz", "md5sum": "75c5063895bccf499a24e43b7423b832", "file_type": "reads", "status": "released", "paired_end": "1", "related_files": [{"relationship_type": "paired with", "file": {"paired_end": "2", "file_type": "reads", "@type": ["FileFastq", "File", "Item"], "status": "released", "accession": "4DNFI4OD4IRB", "file_type_detailed": "reads (fastq)", "display_title": "4DNFI4OD4IRB.fastq.gz", "@id": "/files-fastq/4DNFI4OD4IRB/", "uuid": "4de7ca90-1604-4f5a-a8c3-66f8f5977a46", "file_format": {"@type": ["FileFormat", "Item"], "uuid": "c13d06cf-218e-4f61-aaf0-91f226248b2c", "display_title": "fastq", "status": "released", "@id": "/file-formats/fastq/", "file_format": "fastq", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "external_references": [{"ref": "SRA:SRR18530783", "uri": "https://www.ncbi.nlm.nih.gov/sra/?term=SRR18530783"}], "lab": {"@id": "/labs/yijun-ruan-lab/", "display_title": "Yijun Ruan, JAX", "name": "yijun-ruan-lab", "status": "current", "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "@type": ["Lab", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}, "quality_metric": {"Sequence length": "51", "@type": ["QualityMetricFastqc", "QualityMetric", "Item"], "overall_quality_status": "PASS", "uuid": "8b94fec8-be25-44d6-9fae-9a7a29218ad4", "status": "released", "@id": "/quality-metrics-fastqc/8b94fec8-be25-44d6-9fae-9a7a29218ad4/", "Total Sequences": 90338263, "display_title": "QualityMetricFastqc from 2024-07-02", "url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFI1DK2FFU/fastqc_report.html", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "file_format": {"uuid": "c13d06cf-218e-4f61-aaf0-91f226248b2c", "@id": "/file-formats/fastq/", "@type": ["FileFormat", "Item"], "display_title": "fastq", "file_format": "fastq", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "track_and_facet_info": {"experimental_lab": "Yijun Ruan, JAX", "experiment_type": "ChIP-seq", "experiment_bucket": "raw file", "assay_info": "RNA Pol II", "dataset": "ChIP-seq in HCT116", "condition": "RNAPII antibody - auxin no treatment", "replicate_info": "Biorep 1, Techrep 1", "biosource_name": "HCT116 (Tier 2)", "lab_name": "Yijun Ruan, JAX"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"file_size": 2886060733, "@type": ["FileFastq", "File", "Item"], "accession": "4DNFI4OD4IRB", "file_type_detailed": "reads (fastq)", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/files/4de7ca90-1604-4f5a-a8c3-66f8f5977a46/4DNFI4OD4IRB.fastq.gz", "uuid": "4de7ca90-1604-4f5a-a8c3-66f8f5977a46", "@id": "/files-fastq/4DNFI4OD4IRB/", "href": "/files-fastq/4DNFI4OD4IRB/@@download/4DNFI4OD4IRB.fastq.gz", "file_classification": "raw file", "dbxrefs": ["SRA:SRR18530783"], "display_title": "4DNFI4OD4IRB.fastq.gz", "upload_key": "4de7ca90-1604-4f5a-a8c3-66f8f5977a46/4DNFI4OD4IRB.fastq.gz", "md5sum": "f117419682cdd804d8cf523d461272e8", "file_type": "reads", "status": "released", "paired_end": "2", "related_files": [{"relationship_type": "paired with", "file": {"paired_end": "1", "file_type": "reads", "@type": ["FileFastq", "File", "Item"], "status": "released", "accession": "4DNFI1DK2FFU", "file_type_detailed": "reads (fastq)", "display_title": "4DNFI1DK2FFU.fastq.gz", "@id": "/files-fastq/4DNFI1DK2FFU/", "uuid": "9ca21ce1-c404-4549-8a49-0d96635952d4", "file_format": {"@type": ["FileFormat", "Item"], "uuid": "c13d06cf-218e-4f61-aaf0-91f226248b2c", "display_title": "fastq", "status": "released", "@id": "/file-formats/fastq/", "file_format": "fastq", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "external_references": [{"ref": "SRA:SRR18530783", "uri": "https://www.ncbi.nlm.nih.gov/sra/?term=SRR18530783"}], "lab": {"@id": "/labs/yijun-ruan-lab/", "display_title": "Yijun Ruan, JAX", "name": "yijun-ruan-lab", "status": "current", "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "@type": ["Lab", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}, "quality_metric": {"Sequence length": "51", "@type": ["QualityMetricFastqc", "QualityMetric", "Item"], "overall_quality_status": "PASS", "uuid": "6ebbffce-9969-4094-86ac-5735f2dad8fe", "status": "released", "@id": "/quality-metrics-fastqc/6ebbffce-9969-4094-86ac-5735f2dad8fe/", "Total Sequences": 90338263, "display_title": "QualityMetricFastqc from 2024-07-02", "url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFI4OD4IRB/fastqc_report.html", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "file_format": {"uuid": "c13d06cf-218e-4f61-aaf0-91f226248b2c", "@id": "/file-formats/fastq/", "@type": ["FileFormat", "Item"], "display_title": "fastq", "file_format": "fastq", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "track_and_facet_info": {"experimental_lab": "Yijun Ruan, JAX", "experiment_type": "ChIP-seq", "experiment_bucket": "raw file", "assay_info": "RNA Pol II", "dataset": "ChIP-seq in HCT116", "condition": "RNAPII antibody - auxin no treatment", "replicate_info": "Biorep 1, Techrep 1", "biosource_name": "HCT116 (Tier 2)", "lab_name": "Yijun Ruan, JAX"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "dbxrefs": ["GEO:GSM5983433", "GEO:SRX14660880"], "display_title": "ChIP-seq against RNA Pol II on HCT116 (Tier 2) - 4DNEXACWA3ZI", "accession": "4DNEXACWA3ZI", "@id": "/experiments-seq/4DNEXACWA3ZI/", "biosample": {"treatments_summary": "None", "status": "released", "biosample_category": ["Tier 2"], "@id": "/biosamples/4DNBSQYSWDA1/", "uuid": "b6baa085-d767-4122-b2e0-a974fee17d24", "modifications_summary": "None", "accession": "4DNBSQYSWDA1", "biosource_summary": "HCT116 (Tier 2)", "display_title": "4DNBSQYSWDA1", "@type": ["Biosample", "Item"], "biosample_type": "immortalized cells", "biosource": [{"biosource_type": "immortalized cell line", "display_title": "HCT116 (Tier 2) - 4DNSRMYUIVGD", "@id": "/biosources/4DNSRMYUIVGD/", "status": "released", "cell_line_tier": "Tier 2", "@type": ["Biosource", "Item"], "accession": "4DNSRMYUIVGD", "uuid": "393880ea-f928-406d-a4bf-78435b8975ef", "tissue": {"synonyms": ["large bowel", "posterior intestine"], "@id": "/ontology-terms/UBERON:0001155/", "display_title": "colon", "preferred_name": "colon", "status": "released", "term_id": "UBERON:0001155", "uuid": "3e39f551-bc0b-4bee-b932-29d71956fb94", "term_name": "colon", "@type": ["OntologyTerm", "Item"], "slim_terms": [{"display_title": "large intestine", "@id": "/ontology-terms/UBERON:0000059/", "uuid": "111174bc-8535-4448-903e-854af460a233", "@type": ["OntologyTerm", "Item"], "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"display_title": "digestive system", "@id": "/ontology-terms/UBERON:0001007/", "uuid": "111129bc-8535-4448-903e-854af460a233", "@type": ["OntologyTerm", "Item"], "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "cell_line": {"@id": "/ontology-terms/EFO:0002824/", "synonyms": ["HCT-116 cell", "HCT116 cell"], "status": "released", "@type": ["OntologyTerm", "Item"], "term_name": "HCT116", "uuid": "49d090b2-5326-40ce-9c19-7a214c682272", "term_id": "EFO:0002824", "preferred_name": "HCT116", "display_title": "HCT116", "slim_terms": [{"@id": "/ontology-terms/GO:0005623/", "display_title": "cell", "status": "current", "uuid": "72e16a19-eef3-46ca-a1b8-20e646e69675", "@type": ["OntologyTerm", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"@id": "/ontology-terms/CL:0000000/", "display_title": "cell", "status": "released", "uuid": "45d2b02e-130b-40db-8bf2-2288c6c57dcf", "@type": ["OntologyTerm", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "organism": {"scientific_name": "Homo sapiens", "uuid": "7745b647-ff15-4ff3-9ced-b897d4e2983c", "status": "released", "@id": "/organisms/9606/", "@type": ["Organism", "Item"], "name": "human", "display_title": "H. sapiens", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "tissue_organ_info": {"organ_system": ["large intestine", "digestive system"], "tissue_source": "colon"}, "badges": [{"messages": ["Biosample missing Cell Culture Details"], "badge": {"status": "released", "uuid": "2b2cc7ff-b7a8-4138-9a6c-22884fc71690", "description": "Biosample is missing metadata information required as part of the standards implemented by the 4DN Samples working group.", "badge_icon": "/static/img/badges/biosample-icon.svg", "title": "Biosample Metadata Incomplete", "badge_classification": "Warning", "display_title": "Biosample Metadata Incomplete", "warning": "Biosample Metadata Incomplete", "@type": ["Badge", "Item"], "@id": "/badges/biosample-metadata-incomplete/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "processed_files": [{"@id": "/files-processed/4DNFIA8TIJPG/", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/62a19a8c-83c2-4f69-8ce6-3c472267426c/4DNFIA8TIJPG.bed.gz", "file_type": "read positions", "genome_assembly": "GRCh38", "md5sum": "535447b4982ab0e8f8d8111a231ed5f6", "file_classification": "processed file", "accession": "4DNFIA8TIJPG", "uuid": "62a19a8c-83c2-4f69-8ce6-3c472267426c", "file_type_detailed": "read positions (bed)", "display_title": "4DNFIA8TIJPG.bed.gz", "upload_key": "62a19a8c-83c2-4f69-8ce6-3c472267426c/4DNFIA8TIJPG.bed.gz", "status": "released", "@type": ["FileProcessed", "File", "Item"], "file_format": {"uuid": "69f6d609-f2ac-4c82-9472-1a13331b5ce9", "display_title": "bed", "@id": "/file-formats/bed/", "status": "released", "@type": ["FileFormat", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "href": "/files-processed/4DNFIA8TIJPG/@@download/4DNFIA8TIJPG.bed.gz", "file_size": 1037925806, "quality_metric": {"uuid": "4a75c4ed-822b-442f-a6e4-4d03b823915b", "@type": ["QualityMetricChipseqV2", "QualityMetric", "Item"], "display_title": "QualityMetricChipseqV2 from 2024-09-06", "@id": "/quality-metrics-chipseq-v2/4a75c4ed-822b-442f-a6e4-4d03b823915b/", "status": "released", "url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFIA8TIJPG/qc_report.html", "overall_quality_status": "PASS", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "quality_metric_summary": [{"title": "PCR Bottleneck Coefficient (PBC)", "value": "0.74", "tooltip": "one-read non-mito read pairs / distinct non-mito read pairs", "numberType": "float"}, {"title": "Nonredundant Read Fraction (NRF)", "value": "0.75", "tooltip": "distinct non-mito read pairs / total non-mito read pairs", "numberType": "float"}, {"title": "Filtered & Deduped Reads", "value": "59319734", "numberType": "integer"}]}, "last_modified": {"date_modified": "2024-12-03T17:58:48.340164+00:00"}, "contributing_labs": [{"@id": "/labs/yijun-ruan-lab/", "name": "yijun-ruan-lab", "@type": ["Lab", "Item"], "status": "current", "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "display_title": "Yijun Ruan, JAX", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}], "lab": {"@id": "/labs/4dn-dcic-lab/", "display_title": "4DN DCIC, HMS", "name": "4dn-dcic-lab", "status": "current", "@type": ["Lab", "Item"], "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "track_and_facet_info": {"experimental_lab": "Yijun Ruan, JAX", "experiment_type": "ChIP-seq", "experiment_bucket": "processed file", "assay_info": "RNA Pol II", "dataset": "ChIP-seq in HCT116", "condition": "RNAPII antibody - auxin no treatment", "replicate_info": "Biorep 1, Techrep 1", "biosource_name": "HCT116 (Tier 2)", "lab_name": "4DN DCIC, HMS"}, "external_references": []}], "experiment_type": {"uuid": "47a593da-c458-422a-a974-82b3302e89cb", "@id": "/experiment-types/chip-seq/", "experiment_category": "Sequencing", "display_title": "ChIP-seq", "status": "released", "assay_subclassification": "DNA Binding", "assay_subclass_short": "DNA binding", "assay_classification": "Linear DNA Enrichment", "@type": ["ExperimentType", "Item"], "other_tags": ["1D"], "title": "ChIP-seq", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "last_modified": {"date_modified": "2024-11-06T20:30:44.614410+00:00"}, "experiment_categorizer": {"combined": "Target: RNA Pol II", "field": "Target", "value": "RNA Pol II"}, "external_references": [{"ref": "GEO:GSM5983433", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM5983433"}, {"ref": "GEO:SRX14660880", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=SRX14660880"}]}], "experimentset_type": "replicate", "completed_processes": ["ENCODE_ChIP_Pipeline_2.1.6"], "other_processed_files": [{"type": "archived", "files": [{"@type": ["FileProcessed", "File", "Item"], "file_size": 784007, "file_type_detailed": "peaks (bigbed)", "accession": "4DNFISBQS6M1", "uuid": "869bc21c-739f-40fa-8351-f02a2426b570", "status": "archived", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/869bc21c-739f-40fa-8351-f02a2426b570/4DNFISBQS6M1.bb", "md5sum": "fa817c12448bbcd64829251e1f5b7a1f", "higlass_uid": "f4acd6d9-16b1-41a2-86b7-064fd9a1c236", "file_format": {"display_title": "bigbed", "@id": "/file-formats/bigbed/", "status": "released", "@type": ["FileFormat", "Item"], "uuid": "bbafb7f8-98d5-48d7-8e75-dc40a5186e19", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "genome_assembly": "GRCh38", "@id": "/files-processed/4DNFISBQS6M1/", "file_type": "peaks", "display_title": "4DNFISBQS6M1.bb", "href": "/files-processed/4DNFISBQS6M1/@@download/4DNFISBQS6M1.bb", "quality_metric": {"overall_quality_status": "PASS", "url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFISBQS6M1/qc_report.html", "uuid": "54f9838a-bda0-4f21-a4a7-f8f900fd3ea8", "status": "archived", "@id": "/quality-metrics-chipseq-v2/54f9838a-bda0-4f21-a4a7-f8f900fd3ea8/", "@type": ["QualityMetricChipseqV2", "QualityMetric", "Item"], "display_title": "QualityMetricChipseqV2 from 2024-09-06", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "contributing_labs": [{"@type": ["Lab", "Item"], "status": "current", "name": "yijun-ruan-lab", "@id": "/labs/yijun-ruan-lab/", "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "display_title": "Yijun Ruan, JAX", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}], "static_content": [{"location": "tab:higlass", "description": "auto_generated_higlass_view_config", "content": {"uuid": "01491aee-108f-4899-92f1-3bf3c04f1230", "@id": "/higlass-view-configs/01491aee-108f-4899-92f1-3bf3c04f1230/", "@type": ["HiglassViewConfig", "UserContent", "Item"], "display_title": "4DNFISBQS6M1", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "lab": {"display_title": "4DN DCIC, HMS", "status": "current", "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@type": ["Lab", "Item"], "@id": "/labs/4dn-dcic-lab/", "name": "4dn-dcic-lab", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "last_modified": {"date_modified": "2024-12-04T20:46:28.485368+00:00"}, "track_and_facet_info": {"lab_name": "4DN DCIC, HMS"}}, {"@type": ["FileProcessed", "File", "Item"], "file_size": 784007, "file_type_detailed": "conservative peaks (bigbed)", "accession": "4DNFI3HFH88D", "uuid": "37de3132-fbc6-4aea-a43f-28b62d2c5f97", "status": "archived", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/37de3132-fbc6-4aea-a43f-28b62d2c5f97/4DNFI3HFH88D.bb", "md5sum": "fa817c12448bbcd64829251e1f5b7a1f", "higlass_uid": "1b79ab27-e177-40b0-912e-06ebe2626d6e", "file_format": {"display_title": "bigbed", "@id": "/file-formats/bigbed/", "status": "released", "@type": ["FileFormat", "Item"], "uuid": "bbafb7f8-98d5-48d7-8e75-dc40a5186e19", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "genome_assembly": "GRCh38", "@id": "/files-processed/4DNFI3HFH88D/", "file_type": "conservative peaks", "display_title": "4DNFI3HFH88D.bb", "href": "/files-processed/4DNFI3HFH88D/@@download/4DNFI3HFH88D.bb", "contributing_labs": [{"@type": ["Lab", "Item"], "status": "current", "name": "yijun-ruan-lab", "@id": "/labs/yijun-ruan-lab/", "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "display_title": "Yijun Ruan, JAX", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}], "static_content": [{"location": "tab:higlass", "description": "auto_generated_higlass_view_config", "content": {"uuid": "ae25efd8-895f-43ad-8bff-eb7044de5519", "@id": "/higlass-view-configs/ae25efd8-895f-43ad-8bff-eb7044de5519/", "@type": ["HiglassViewConfig", "UserContent", "Item"], "display_title": "4DNFI3HFH88D", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "lab": {"display_title": "4DN DCIC, HMS", "status": "current", "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@type": ["Lab", "Item"], "@id": "/labs/4dn-dcic-lab/", "name": "4dn-dcic-lab", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "last_modified": {"date_modified": "2024-12-04T20:46:27.320737+00:00"}, "track_and_facet_info": {"lab_name": "4DN DCIC, HMS"}}, {"@type": ["FileProcessed", "File", "Item"], "file_size": 1238021899, "file_type_detailed": "signal fold change (bw)", "accession": "4DNFISTG17AB", "uuid": "7bd7cdfc-a2dc-453d-b350-bcdb65278979", "status": "archived", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/7bd7cdfc-a2dc-453d-b350-bcdb65278979/4DNFISTG17AB.bw", "md5sum": "27fb074094cede8a09929aefb61c47a4", "higlass_uid": "049bd03c-df42-4b73-ba68-ec8b9fb4d5c9", "file_format": {"display_title": "bw", "@id": "/file-formats/bw/", "status": "released", "@type": ["FileFormat", "Item"], "uuid": "d1311111-218e-4f61-aaf0-91f226248b2c", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "genome_assembly": "GRCh38", "@id": "/files-processed/4DNFISTG17AB/", "file_type": "signal fold change", "display_title": "4DNFISTG17AB.bw", "href": "/files-processed/4DNFISTG17AB/@@download/4DNFISTG17AB.bw", "contributing_labs": [{"@type": ["Lab", "Item"], "status": "current", "name": "yijun-ruan-lab", "@id": "/labs/yijun-ruan-lab/", "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "display_title": "Yijun Ruan, JAX", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}], "static_content": [{"location": "tab:higlass", "description": "auto_generated_higlass_view_config", "content": {"uuid": "e79dd90b-eb1e-477f-a61e-b4c60f40ca5e", "@id": "/higlass-view-configs/e79dd90b-eb1e-477f-a61e-b4c60f40ca5e/", "@type": ["HiglassViewConfig", "UserContent", "Item"], "display_title": "4DNFISTG17AB", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "lab": {"display_title": "4DN DCIC, HMS", "status": "current", "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@type": ["Lab", "Item"], "@id": "/labs/4dn-dcic-lab/", "name": "4dn-dcic-lab", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "last_modified": {"date_modified": "2024-12-04T20:46:26.123493+00:00"}, "track_and_facet_info": {"lab_name": "4DN DCIC, HMS"}}], "title": "4DN-DCIC ChIP-Seq Pipeline - Archived Files", "description": "These files were generated by the 4DN-DCIC ChIP-seq pipeline without using the control input experiments that were provided. The pipeline was re-run with the controls and those files are available in the processed files tab. These files have been archived.", "higlass_view_config": {"display_title": "4DNESKLX2LUW - 4DN-DCIC ChIP-Seq Pipeline - Archived Files", "description": "Supplementary Files (4DN-DCIC ChIP-Seq Pipeline - Archived Files) for 4DNESKLX2LUW (ChIP-seq in HCT116-RAD-mAC using antibody RNAPII treated with auxin-0h): 4DNFISBQS6M1, 4DNFI3HFH88D, 4DNFISTG17AB", "@type": ["HiglassViewConfig", "UserContent", "Item"], "@id": "/higlass-view-configs/8b14b485-afc8-4c4e-aff8-e63a50ca9819/", "uuid": "8b14b485-afc8-4c4e-aff8-e63a50ca9819", "status": "released", "last_modified": {"date_modified": "2024-11-21T06:07:36.757278+00:00"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "@id": "/experiment-set-replicates/4DNESKLX2LUW/", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "uuid": "c9b222ea-9dc9-498e-a876-e0ba3eb35c2b", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "4DNESKLX2LUW", "external_references": [], "produced_in_pub": {"abstract": "Cohesin is required for chromatin loop formation. However, its precise role in  regulating gene transcription remains largely unknown. We investigated the  relationship between cohesin and RNA Polymerase II (RNAPII) using single-molecule  mapping and live-cell imaging methods in human cells. Cohesin-mediated  transcriptional loops were highly correlated with those of RNAPII and followed  the direction of gene transcription. Depleting RAD21, a subunit of cohesin,  resulted in the loss of long-range (>100 kb) loops between distal  (super-)enhancers and promoters of cell-type-specific genes. By contrast, the  short-range (<50 kb) loops were insensitive to RAD21 depletion and connected  genes that are mostly housekeeping. This result explains why only a small  fraction of genes are affected by the loss of long-range chromatin interactions  due to cohesin depletion. Remarkably, RAD21 depletion appeared to up-regulate  genes located in early initiation zones (EIZ) of DNA replication, and the EIZ  signals were amplified drastically without RAD21. Our results revealed new  mechanistic insights of cohesin's multifaceted roles in establishing  transcriptional loops, preserving long-range chromatin interactions for  cell-specific genes, and maintaining timely order of DNA replication.", "@id": "/publications/a84d089a-b2a0-4da5-a454-07f73987df08/", "display_title": "Kim M et al. (2024) doi:10.1101/2024.03.25.586715", "short_attribution": "Kim M et al. (2024)", "uuid": "a84d089a-b2a0-4da5-a454-07f73987df08", "status": "current", "url": "https://www.ncbi.nlm.nih.gov/pubmed/38585764", "title": "Multifaceted roles of cohesin in regulating transcriptional loops.", "date_published": "2024-03-27", "ID": "doi:10.1101/2024.03.25.586715", "@type": ["Publication", "Item"], "authors": ["Kim M", "Wang P", "Clow PA", "Chien IE", "Wang X", "Peng J", "Chai H", "Liu X", "Lee B", "Ngan CY", "Yue F", "Milenkovic O", "Chuang JH", "Wei CL", "Casellas R", "Cheng AW", "Ruan Y"], "journal": "bioRxiv : the preprint server for biology", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "pubs_using": [], "publications_of_set": [{"journal": "bioRxiv : the preprint server for biology", "date_published": "2024-03-27", "@type": ["Publication", "Item"], "status": "current", "authors": ["Kim M", "Wang P", "Clow PA", "Chien IE", "Wang X", "Peng J", "Chai H", "Liu X", "Lee B", "Ngan CY", "Yue F", "Milenkovic O", "Chuang JH", "Wei CL", "Casellas R", "Cheng AW", "Ruan Y"], "display_title": "Kim M et al. (2024) doi:10.1101/2024.03.25.586715", "uuid": "a84d089a-b2a0-4da5-a454-07f73987df08", "abstract": "Cohesin is required for chromatin loop formation. However, its precise role in  regulating gene transcription remains largely unknown. We investigated the  relationship between cohesin and RNA Polymerase II (RNAPII) using single-molecule  mapping and live-cell imaging methods in human cells. Cohesin-mediated  transcriptional loops were highly correlated with those of RNAPII and followed  the direction of gene transcription. Depleting RAD21, a subunit of cohesin,  resulted in the loss of long-range (>100 kb) loops between distal  (super-)enhancers and promoters of cell-type-specific genes. By contrast, the  short-range (<50 kb) loops were insensitive to RAD21 depletion and connected  genes that are mostly housekeeping. This result explains why only a small  fraction of genes are affected by the loss of long-range chromatin interactions  due to cohesin depletion. Remarkably, RAD21 depletion appeared to up-regulate  genes located in early initiation zones (EIZ) of DNA replication, and the EIZ  signals were amplified drastically without RAD21. Our results revealed new  mechanistic insights of cohesin's multifaceted roles in establishing  transcriptional loops, preserving long-range chromatin interactions for  cell-specific genes, and maintaining timely order of DNA replication.", "ID": "doi:10.1101/2024.03.25.586715", "@id": "/publications/a84d089a-b2a0-4da5-a454-07f73987df08/", "title": "Multifaceted roles of cohesin in regulating transcriptional loops.", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "number_of_experiments": 1, "@context": "/terms/", "aggregated-items": {"badges": [{"parent": "/biosamples/4DNBSQYSWDA1/", "embedded_path": "experiments_in_set.biosample.badges", "item": {"messages": ["Biosample missing Cell Culture Details"], "badge": {"commendation": null, "warning": "Biosample Metadata Incomplete", "uuid": "2b2cc7ff-b7a8-4138-9a6c-22884fc71690", "@id": "/badges/biosample-metadata-incomplete/", "badge_icon": "/static/img/badges/biosample-icon.svg", "description": "Biosample is missing metadata information required as part of the standards implemented by the 4DN Samples working group."}}}, {"parent": "/experiment-set-replicates/4DNESKLX2LUW/", "embedded_path": "badges", "item": {"messages": ["Replicate set contains only a single biological replicate"], "badge": {"commendation": null, "warning": "Replicate Numbers", "uuid": "24a64a84-3c33-4d76-aaf2-e5ef45eff347", "@id": "/badges/replicate-numbers/", "badge_icon": "/static/img/badges/replicates-orange-circle.svg", "description": "Issues with replicate numbers"}}}]}, "validation-errors": []}