{"lab": {"status": "current", "display_title": "Benoit Bruneau, UCSF", "@type": ["Lab", "Item"], "uuid": "4dfac8f5-a868-4b29-9c11-a0932130e121", "@id": "/labs/benoit-bruneau-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.4dfac8f5-a868-4b29-9c11-a0932130e121"]}}, "url": "https://www.addgene.org/86230/", "name": "pEN84-CTCF-AID-eGFP", "award": {"status": "current", "display_title": "EXTERNAL AWARD", "uuid": "12a92962-8265-4fc0-b2f8-cf14f05db58b", "@type": ["Award", "Item"], "@id": "/awards/external-award/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "status": "released", "aliases": ["4dn-dcic-lab:construct_pEN84-CTCF-AID-eGFP"], "description": "Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system. Designed to be used with sgRNA ATCACCGGTCCATCATGCTG and CTGGGGCCTTGCTCGGCACC for Ctcf targeting. Plasmid # pEN84 created by Elphege Nora. Addgene cat # 86230.", "date_created": "2022-07-11T09:41:26.382230+00:00", "protein_tags": ["AID", "eGFP"], "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2022-07-11T09:45:20.644386+00:00"}, "construct_type": "crispr construct", "public_release": "2022-07-11", "schema_version": "1", "project_release": "2022-07-11", "vector_backbone": "pFNF (Addgene #22687)", "construct_vendor": {"@id": "/vendors/addgene/", "display_title": "Addgene", "@type": ["Vendor", "Item"], "status": "released", "uuid": "ab511678-a44d-4312-a71f-633f3e07be52", "title": "Addgene", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "designed_to_target": "CTCF", "@id": "/constructs/96b490b1-ce1a-49bd-8bc8-055143435c58/", "@type": ["Construct", "Item"], "uuid": "96b490b1-ce1a-49bd-8bc8-055143435c58", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "pEN84-CTCF-AID-eGFP", "external_references": [], "@context": "/terms/", "aggregated-items": {}, "validation-errors": []}