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8 Common Properties
 Project
4DN
 Organism
Human
 Sample Type
immortalized cells
 Sample Tier
Tier 2
 Sample Category
Tier 2
 Tissue Source
retinal pigment epithelial cell
 Modifications
No value
 Treatments
No value
Title
Experiment Type
Biosample
Dataset
Condition
Assay Details
Lab
multiplexed FISH
RPE-hTERT (Tier 2)
Chromatin tracing of chrX
RPE-1 cells, untreated
Target
28 consecutive 30-kb loci on chrX, mH2A.1
DamID-seq
RPE-hTERT (Tier 2)
DamID-seq on RPE cells (2020-07-01)
Dam-only control
Target
None (Control)
DamID-seq
RPE-hTERT (Tier 2)
DamID-seq on RPE cells (2020-07-01)
Nucleolar Targeting Signal (4X-AP3)-Dam
Target
Nucleolus Localization Sequence (4X-AP3D1) (engineered reagent)
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminB1 antibody
Target
LMNB1 protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
Dam control for normalization
-
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
Dam control for normalization - synchronized 21h after mitotic shakeoff
-
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
Dam control for normalization - synchronized 10h after mitotic shakeoff
-
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
Dam control for normalization - synchronized 6h after mitotic shakeoff
-
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
Dam control for normalization - synchronized 3h after mitotic shakeoff
-
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
Dam control for normalization - synchronized 1h after mitotic shakeoff
-
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminB2 antibody - synchronized 21h after mitotic shakeoff
Target
LMNB2 protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminB2 antibody - synchronized 10h after mitotic shakeoff
Target
LMNB2 protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminB2 antibody - synchronized 6h after mitotic shakeoff
Target
LMNB2 protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminB2 antibody - synchronized 3h after mitotic shakeoff
Target
LMNB2 protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminB2 antibody - synchronized 1h after mitotic shakeoff
Target
LMNB2 protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminAC antibody - attached cells
Target
LaminA/C protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminB2 antibody - attached cells
Target
LMNB2 protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
Dam control for normalization - attached cells
-
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminAC antibody
Target
LaminA/C protein
pA-DamID
RPE-hTERT (Tier 2)
pADamID on RPE
LaminB2 antibody
Target
LMNB2 protein
DamID-seq
RPE-hTERT (Tier 2)
DamID-seq on RPE cells (2018-10-22)
Dam-only Control
Target
None (Control)
DamID-seq
RPE-hTERT (Tier 2)
DamID-seq on RPE cells (2018-10-22)
LaminB1-Dam
Target
LMNB1 protein
2-stage Repli-seq
RPE-hTERT (Tier 2)
E/L repliseq on RPE-1 hTERT cells (2017-08-17)
late
Fraction
late fraction of 2 fractions
2-stage Repli-seq
RPE-hTERT (Tier 2)
E/L repliseq on RPE-1 hTERT cells (2017-08-17)
early
Fraction
early fraction of 2 fractions
- fin -